MLTE 221 Medical Laboratory Microbiology

This course introduces basic practices and principles of diagnostic microbiology, focusing on pathogenic bacteria encountered in the blood, central nervous system, and genitourinary tract. It includes application of common algorithms for identification of clinically significant pathogens including aerobic gram-positive cocci, gram-negative bacilli, gram-negative cocci, gram-positive bacilli, and anaerobes. The course introduces principles and procedures of immunological and molecular diagnostic techniques and their application to the medical lab.

Semester Contact Hours Lecture

60

Semester Contact Hours Lab

0

MLTE 221Medical Laboratory Microbiology

Please note: This is not a course syllabus. A course syllabus is unique to a particular section of a course by instructor. This curriculum guide provides general information about a course.

I. General Information

Department

Health Science and Human Services Technical

II. Course Specification

Course Type

{D7A8FC71-978F-4003-9933-512C476323B2}

Semester Contact Hours Lecture

60

Semester Contact Hours Lab

0

III. Catalog Course Description

This course introduces basic practices and principles of diagnostic microbiology, focusing on pathogenic bacteria encountered in the blood, central nervous system, and genitourinary tract. It includes application of common algorithms for identification of clinically significant pathogens including aerobic gram-positive cocci, gram-negative bacilli, gram-negative cocci, gram-positive bacilli, and anaerobes. The course introduces principles and procedures of immunological and molecular diagnostic techniques and their application to the medical lab.

IV. Student Learning Outcomes

Upon completion of this course, a student will be able to:

• Explain safe laboratory protocols, including sterile techniques, disinfection of the work areas, and biohazardous spill clean-up, with appropriate use of each technique in a given circumstance.
• Discuss the medically significant bacterial microorganisms.
• Explain and utilize the various identification techniques used in the medical laboratory.
• Differentiate between different genera of organisms within a gram stain group by biochemical tests and reactions, i.e. staphylococcus or streptococcus.
• Identify organisms by standard characteristics of the genera or group, i.e. staphylococci, streptococci, common Enterobacteriaceae, Pseudomonas, Neisseria, Haemophilus, Bacillus, diphtheroids, etc.
• Differentiate appropriate organisms to the species, i.e. Staphylococcus aureus, Staphylococcus epidermidis.
• Explain the principles and the resulting reactions of a variety of standard biochemical tests used in organism identificationschemes.
• Describe quality control procedures used in the Microbiology department.
• Differentiate fermentative and non-fermentative gram negative bacilli.
• Define different atmospheric growth conditions required by fastidious organisms such as Neisseria sp., Haemophilus sp., andCampylobacter.
• Differentiate aerobic and anaerobic organisms in culture.
• Recognize and correlate gram morphology to genus names of anaerobic organisms.
• Explain the principles of antimicrobial susceptibility testing, including the standardization protocols and the interpretation of test results.
• Demonstrate the use of reference materials in susceptibility testing, regarding special situations such as MRSA and penicillin resistant Streptococcus pneumonia, and Haemophilus influenzae susceptibility.

V. Topical Outline (Course Content)

VI. Delivery Methodologies